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Platform > Biology, Molecular Biology, Ecophysiology

Molecular biology (Bouloie)

B2ME Technical Plole

Managers:
GOYDADIN Anne-Claude->spip.php?page=perso&nom=GOYDADIN&prenom=Anne-Claude]
Office: -209M - Phone: +33 3 81 66 57 91
Mail: GOYDADIN Anne-Claude

BORNE Romain
Bureau: -211M - Tél: +33 3 81 66 58 29
Mail: BORNE Romain

Location
La Bouloie - propédeutique building - building wing M - floor -2 (-20xM)

Brief summary of the activity
The technical unit of molecular biology is intended for the manipulation of plant, animal and environmental samples for the study of molecular processes or diagnostics involving DNA, RNA or protein molecules (extraction, amplification, purification, etc.). The rooms and equipments are organized in a forward configuration in order to limit cross-contaminations.

The analyzes or preparations

  • Bacterial culture
  • DNA/RNA extraction
  • Concentration assay of DNA/RNA/Protein
  • Conventional PCR of DNA/RNA
  • qPCR of DNA/RNA

List of devices

  • Bacterial culture
    • Hyridation Oven /shaker SI20H
    • Orbitale shaker Format Scientific
    • Benchtop autoclave Advantage Lab AL 02-01
    • Incubator BINDER
  • Nucleic acid extraction
    • Tissuelyser II (Qiagen)
    • Thermomixer Eppendorf
    • Extractor (sorbonne)
    • Dry bath incubator Grant
    • Concentrator 5301 Eppendorf
    • Automate of Qiagen spin-column kits QIAcube (Qiagen)
  • Concentration assay of nucleic acids and proteins
    • Fluorometer Quantus Promega
    • Spectrophotometer NanoVue Plus Bbiochrom
    • Spectrophotometer biophotometer Eppendorf
  • Conventional PCR
    • Thermocycler Eppendorf Mastercycler nexus Gradient
    • Thermocyler SimpliAmp Applied Biosystems Lifetechnologies
    • PCR extractor
  • qPCR ADN/ARN
    • qPCR themocycler Eppendorf Mastercycle Realplex2 EpgradientS
  • Electrophoresis
    • Capillary electrophoresis automaton Qiaxcel (Qiagen)
    • Agarose gel tanks / Electrophoresis generator BioRad
    • GelDoc™ XR+ and Imaging Lab Software (BIORAD)

Access conditions:
The equipment can be used freely after training by a supervisor.

Contact:
GOYDADIN Anne-Claude
BORNE Romain

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