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Research > Technological Platform for the Study of Old and Present Environments (PEA²t) > Common facilities

Centrifugation, laundry, UP and osmosis water production, drying, lyophilizer, autoclave

SC Technical Platform

Manager

AMIOT Caroline
Office: -203M - Phone : +33 3 81 66 57 83
Mail: AMIOT Caroline

Centrifugation

Location

  • Centrifuge 5430R Eppendorf : room -219M
  • Centrifuge 5301 Eppendorf : room -219M
  • Centrifuge 5415 Eppendorf : room -205M
  • Centrifuge SIGMA 4-16 : room -225M
  • Centrifuge HERAEUS Biofuge PRIMO : room -204K
  • 2 Centrifuges SORVALL ST16 : room -220M
  • Centrifuge HETTICH Universal 320 : room -103K
  • Centrifuge Beckman Coulter AVANTI J-25 : room -226M

Brief summary of the activity
The centrifugation is a process of separation of the compounds of a mixture by submitting them to a centrifugal force. She allows the “recovery” of a precipitate, the separation of elements represented in the blood (red blood cells, white blood cells, plaques in suspension in the blood plasma), the separation of cellular compounds, the desiccation of muds for the waste water treatment.

List of devices

  • PCR tubes
  • ADN, ARN, protein, blood
  • Chemicals
  • Water

Laundry

Location
La Bouloie - Propédeutique building - Building wing M - Floor -2 (-218M)

Brief summary of the activity
Standard or intensive wash of glasseware laboratory (various possible washing programs)

List of devices
Laveur de laboratoire MIELE PG8593

UP and osmosis water production

Location
La Bouloie - Propédeutique building - Building wing M - Floor -4 (Ultrapure water production room)

Brief summary of the activity
Production of osmosed water (type II) or ultrapure water (type I)

List of devices
Milli Q Integral 3 MERCK MILLIPORE capacity of production of ultrapure water 70l/j , maximum flow 0.05 to 2L/minute resistivity 18.2MΩ.cm, COT<5ppb ; capacity of production of osmosed water 70l/j , maximum flow 2L/minute, resistivity >5MΩ.cm, COT<30ppb
Elix/Synergy MILLIPORE capacity of production of ultrapure water 60l/j resisitivity 18 MΩ.cmcapacity of production of osmosed water 60l/j maximum flow 90l/h resistivity 8 MΩ.cm
Equipment : capacity of production 350l/h resisitivity 18.2 MΩ.cm, COT<5ppb
Access conditions:

Drying, lyophilizer

Location

  • Freeze dryers : -308M
  • Stoves : -308M (another one also in room -210M but in UT preparation grounds and vegetation)

Brief summary of the activity
Preparation of samples of all kinds (muds, sediments, grounds, vegetation, animal tissues) by drying.
The drying can take place in a stove (in this case, the volatile elements such as the mercury will be lost), either by freeze-drying. This technique allows to keep the shape and the aspect of samples and facilitates the operation of grinding.

The analyzes or preparations

  • Freeze-drying : vacuum dehydration by sublimation of a product (solid, pasty or liquid) beforehand frozen in -80°C (a freezer in 80°C is also in the room) : the steam leaves the product and is captured by means of a cold trap. The time of drying is of 48 hours on average but can vary from 24 to 72 hours according to the nature or the size of samples.
  • Drying or mineralization of samples, range of temperature of 0 in 200° C. The time of drying can vary from 12 to 72 hours according to the nature or the size of samples.

List of devices

  • Freezedryer CRYOTEC Cosmo 20K with vacuum pump Adixen serie C1
  • Freezedryer FREEZEMOBILE 6
  • Stove RAYPA DOD-150
  • Stove JOUAN
  • Stove MEMMERT (UT preparation grounds and vegetation)

Access conditions:
The room is accessible via the booking portal of the laboratory’s PES platform.

To the reservation portal (restricted area)

Autoclave

Location
Besançon - La Bouloie site - Building wing M - Floor -2 - Room -218M

Brief summary of the activity
An autoclave is a device of laboratory allowing to realize the sterilization, under pressure, of objects of full shape, hollow, porous, packed or not packed. He allows then to use the object sterilized without any risk of contamination.
So that an object of laboratory is completely sterile, it is necessary to eliminate all the germs. Autoclaves allow to destroy the most virulent microorganisms, but must also be rather flexible to protect the integrity of components, products and packagings. A big range of cycles of sterilization increases the versatility of processing.

The analyzes or preparations
Allows to realize fast solid or liquid cycles of sterilization in 121°C or 134°C

List of devices
Autoclave PROHS 150

Contact:
AMIOT Caroline

 Centrifugation, laundry, UP and osmosis water production, drying, lyophilizer, autoclave

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