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Research > Technical Facilities > Environmental Chimical Analysis

Chromatography

Plateau technique ACE

Chromatography - Besançon lab.

Managers:
CRINI Nadia (IR)
Bureau: -203M - Tél: +33 3 81 66 57 86
Mail: CRINI Nadia

AMIOT Caroline (Technicienne)
Bureau: -203M - Tél: +33 3 81 66 57 83
Mail: AMIOT Caroline

Location
Besançon - La Bouloie - Bâtiment Propédeutique - aile M - étage -2 (-204M)

Brief summary of the activity

  • Extraction, separation, identification and quantification of organic contaminants from various matrices (water, soil, sediment, biological tissues) with analytical chemistry tools for support of scientific research programs in environment and health-relevant areas.
  • Steady development of analytical methods for the measurement of organic compounds in novel matrices; characterization and transfer in the food chain.

The analyzes or preparations
Extraction:

  • Liquid liquid
  • Pressurized liquid extraction (PLE)
  • Microwave
  • QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe)
  • Soxhlet: under development
  • Solid phase extraction (SPE): under development

Purification:

  • Column or cartridge (with silica gel, florisil® …) of purification: under development
  • Analysis:
  • 16 Polycyclic aromatic hydrocarbons (PAH):
  • Naphtalene, Acenaphtylene, Acenaphtene, Fluorene, Phenanthrene, Anthracene, Fluoranthene, Pyrene, Benzo(a)anthracene, Chrysene, Benzo(b)fluoranthene, Benzo(k)fluoranthene, Benzo(a)pyrene, Indeno(1,2,3-cd)pyrene, Dibenzo(ah)anthracene, Benzo(ghi)perylene
  • Applications: water, effluents, soils, sediments, vegetation, manure/liquid manure, milk, biological tissues such as blood, snails, liver raptor for example
  • 11 Polychlorinated biphenyls (PCB) : PCB 28, 52, 77, 101, 138, 153, 180, 118, 126, 169 et 194. Applications: water, effluents, soils, sediments
  • 5 triazole fungicides: Propiconazole, tebuconazole, epoxiconazole, bromuconazole, difenoconazole. Applications: water, soils, sediments.

List of devices

  • Triple quadrupole gas chromatography (GC-MS/MS) Agilent (GC System 7890A, GC MS Triple Quad 7000), electron impact source, with automatic sampler Combipal (GC Sampler 80) and nitrogen gas generator unit Gengaz (NiGen LCMS 40-1)
  • Pressurized liquid extraction apparatus (PLE) with 40 or 80 ml cells Buchi (Speed Extractor E-914) and nitrogen generator Gengaz (Ni Gen LCMS 40-1)
  • Soxhlet
  • 12 positions-evaporator/concentrator with residual volume Buchi (Syncore)
  • Microwave extractor with automatic sampler (MAS24) Anton Paar (Monowave 450) : being acquired
 Chromatography - Besançon lab.
Chromatography - Besançon lab.

Chromatography - Montbéliard lab.

Manager:
TOUSSAINT Marie-Laure
Office: - Tél: +33 3 81 66 57 83
Mail: TOUSSAINT Marie-Laure

Location
Montbéliard - STGI-B building - Floor 2 (B1)

Brief summary of the activity
In the Montbéliard campus, STGi-B building, st.2, room B1
Extraction and GC-MS analysis of organic compounds in soil or plant samples.

The analyzes or preparations

  • Hot solvent extraction under 100 bar pressure on lyophilized and ground samples.
  • Concentration and possible purification of the extract.
  • Quantification by gas chromatography of PAH compounds and some pesticides.

List of devices

  • GC-MS Shimadzu QP 5050A (1999), single mass detector
  • Dionex ASE 100 Extractor (2006), manual mode.
  • Biotage TurboVap II (2012), evaporation under nitrogen.
  • VacMaster, Biotage (2012), support for 10 SPE column.

Contact:
TOUSSAINT Marie-Laure

 Chromatography - Montbéliard lab.
Chromatography - Montbéliard lab.

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