Cyril Zappelini is pleased to invite you to his thesis defense entitled "Study of Rhizospheric microbial communities of woody native to anthropogenic soils, derived from industrial effluents". It will be held Tuesday, July 3 at 14 pm, in the amphitheater of building B, University Pole of the Pays de Montbéliard.

Jury members:

• Vera SLAVEYKOVA (Professor, University of Geneva) Rapporteur
• Bertrand AIGLE (Professor, University of Lorraine) Rapporteur
• Céline ROOSE-AMSALEG (IGR, University of Rennes) Examiner
• Karine JEZEQUEL (Lecturer, University of Haute Alsace) Examiner
• Nicolas CAPELLI (Lecturer HDR, UBFC) Supervisor
• Christophe GUYEUX (Professor, UBFC) Thesis co-supervisor
• Michel CHALOT (Professor, UBFC) Thesis director

Abstract

My thesis subject includes one of the global projects of the UMR UFC/CNRS 6249 Chrono-environnement entitled “phytoremediation strategies based on the use of trees and associated microorganisms”, which is based, among other things, on 2 research projects:

• the PROLIPHYT project (Eco-Industry programme, 2013-2018, ADEME) entitled « Production of woody phytoremediants »,
• the PHYTOCHEM project (ANR CD2i, 2013-2018) entitled “Development of eco-innovative chemical processes to exploit biomasses from phytotechnologies”.
  The general objectives are to improve the phytoremediation potential of a panel of woody species and to develop the microbial potential for assisted phytoremediation on contaminated soil. In addition to limiting the impact of pollutants, this strategy aims to promote the production of biomass on land abandoned and not exploitable by agriculture, while ensuring the biodiversity needed to restore an anthropogenic ecosystem. My thesis work is financed through a ministerial doctoral contract for disability (dyslexia). It is based on the rehabilitation of two industrial sediment storage areas, used until the 2000s. These two experimental sites (INOVYN site of Saint-Symphorien-sur-Saône in Côte-d’Or, CRISTAL site of Ochsenfeld in Alsace) present very particular physico-chemical characteristics which make them privileged places of study. The first is a former settling lagoon whose sediments enriched in Hg, Ba and As come from the treatment of wastewater from SOLVAY’s Hg electrolysis process. The second is a lagoon consisting of a backfill in which the titanium dioxide extraction residues from the CRISTAL Thann Plant have been stored since the 1930s. In contrast to the first experimental site, there is a low abundance of flora which results in heterogeneous development of a main woody species, the birch. The natural and spontaneous recolonisation of plants, more particularly woody species on both sites, is undoubtedly the result of close collaboration with telluric microorganisms located near their root systems. We have thus chosen to work on 3 pioneer species that have naturally relocated to the two study sites: willow and poplar for the industrial wasteland of Tavaux and birch for the effluent treatment unit at the Ochsenfeld site.

Keywords :
Tailings (anthropogenic soil), natural environment (undisturbed soil), Salicaceae (poplar, willow), Betulaceae (birch), rhizobacteria, metabarcoding, diversity, identification, characterization, Plant Growth Promoting Rhizobacteria, environmental microbiology, INOVYN site of Tavaux, Ochsenfeld site.