Chromatography

PEA²t : Environmental chemical analysis (ACE)

Chromatography

The Chromatography technical unit of the UMR Chono-environment offers two types of activity:

1 – Extraction, separation, identification and quantification of organic contaminants in various matrices (water, soil, sediment, biological tissue), using analytical chemistry tools, to support research programmes in the field of the Environment and Health.
2 – Regular development of methods for measuring new organic compounds in original matrices; characterisation and tracing of their transfer into the food chain.

Analyses or preparations

Extraction :
– Liquid liquid
– Pressurised liquid (PLE)
– Microwave
– QuEChERS
– Soxhlet: under development
– Solid phase (SPE): under development
Purification :
– Silica gel purification columns or cartridges, florisil®…: under development
– Analysis :
– 16 polycyclic aromatic hydrocarbons (PAHs):
– Naphthalene, Acenaphthylene, Acenaphthene, Fluorene, Phenanthrene, Anthracene, Fluoranthene, Pyrene, Benzo(a)anthracene, Chrysene, Benzo(b)fluoranthene, Benzo(k)fluoranthene, Benzo(a)pyrene, Indeno(1,2,3-cd)pyrene, Dibenzo(ah)anthracene, Benzo(ghi)perylene
– Applications: water, effluents, soil, sediments, vegetation, manure, milk, biological tissues such as blood, snails, raptor liver, etc.
– 11 polychlorinated biphenyls (PCBs): PCB 28, 52, 77, 101, 138, 153, 180, 118, 126, 169 and 194. Applications: water, effluents, soil, sediments, etc.
– 5 triazole fungicides: Propiconazole, tebuconazole, epoxiconazole, bromuconazole, difenoconazole. Applications: water, soil, sediment.

List of equipment

– Agilent triple quadrupole chromatography (GC-MS/MS) (GC System 7890A, GC MS Triple Quad 7000), electron impact ionisation source, equipped with a Combipal sample changer (GC Sampler 80) and powered by a Gengaz nitrogen generator (NiGen LCMS 40-1)
– Pressurised Liquid Extractor (PLE) fitted with 40 or 80 ml Buchi cells (Speed Extractor E-914) and equipped with a Gengaz nitrogen generator (Ni Gen LCMS 40-1)
– Soxhlet extractor
– 12-station evaporator/concentrator with Buchi residual volume (Syncore)
– Anton Paar (Monowave 450) microwave extractor fitted with an autosampler (MAS24): currently being acquired

Chromatography in Montbéliard

On the Montbéliard campus, STGi-B building, stage 2, room B1
Extraction and analysis by GC-MS of organic compounds in soil and plant samples.

Analyses or preparations

– Hot solvent extraction under 100 bar pressure on freeze-dried and ground samples.
– Concentration and possible purification of the extract.
– Gas chromatographic quantification of PAH compounds and certain pesticides.

List of equipment

– GC-MS Shimadzu QP 5050A (1999), single mass detector.
– Dionex ASE 100 extractor (2006), manual mode.
– Biotage TurboVap II (2012), evaporation under nitrogen.
– VacMaster, Biotage (2012), support for 10 SPE columns.